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C (5% CO2) for the designated time period. Avant Garde Premium Thai Kratom Trout the adherent cells (HEK 293 and SH-SY5Y cells) were harvested trypsinised and centrifuged as per routine procedures described in chapter 2 sections 2. After this incubation the cells were harvested as previously described (section 2.
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Q3 (%) 10. Table show values of triplicate reading of each quadrant from 3 similar experiments. Programmed cell death or apoptosis is one way cells can commit to death induced by numerous factors. In the present study a possible involvement of caspase proteases both pro-apoptotic caspases (caspase 8 and 9) and executor caspases (caspase 3 and 7) were examined using commercially available kits as described in section 5. Possible involvement of pro-apoptotic caspases (8 and 9) The caspase 8 colorimetric assay performed
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on SH-SY5Y cell thai kratom powder review lysates indicated little difference between all MSE treated groups and control group for both 4 hr and 24 hr incubation time period (Fig. A and B). The same outcome was also noted for caspase 9 assay which was performed using the same cell lysates (Fig.
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